Efficacy of Seed Treatment and Foliar Application of New-Generation Fungicides for the Management of Anthracnose Caused by Colletotrichum Truncatum in Soybean

Priyanshi Raghuwanshi; Pawan Kumar Amrate; M. K. Shrivastava

doi:10.53550/sr.v23.i2.117

Loading article…

Loading page…

Efficacy of Seed Treatment and Foliar Application of New-Generation Fungicides for the Management of Anthracnose Caused by Colletotrichum Truncatum in Soybean

Priyanshi Raghuwanshi, Pawan Kumar Amrate, M. K. Shrivastava

DOI: 10.53550/sr.v23.i2.117

Published 9/17/2026

PDF4.7 MB

Abstract

Anthracnose caused by Colletotrichum truncatum (Schw.) Andrus and Moore is one of the most destructive diseases of soybean in India, resulting in considerable yield losses. The present investigation was conducted during Kharif 2023 at JNKVV, Jabalpur (Madhya Pradesh), to evaluate the field efficacy of four fungicides (eight treatments) applied as seed treatment (ST), foliar spray (FS), and their combinations for the management of soybean anthracnose. All fungicidal treatments significantly reduced disease severity, disease incidence, pod infection and Area Under Disease Progress Curve (AUDPC), while improving plant health and grain yield over the untreated control. Among the treatments, T8 [ST Penflufen 13.28% w/w + Trifloxystrobin 13.28% w/w @ 1.25 ml kg⁻¹ seed + FS Tebuconazole 10% + Sulphur 65% WG @ 2 g l⁻¹ water] recorded the lowest anthracnose severity (5.37%) compared with the untreated control (23.33%). This treatment was followed by T7 [ST Penflufen 13.28% w/w + Trifloxystrobin 13.28% w/w @ 1.25 ml kg⁻¹ seed + FS Carbendazim 12% + Mancozeb 63% @ 2 g l⁻¹ water] (5.93%) and T6 [ST Carboxin 37.5% + Thiram 37.5% DS @ 2.5 g kg⁻¹ seed + FS Tebuconazole 10% + Sulphur 65% WG @ 2 g l⁻¹ water] (6.76%), which were statistically at par with T8. The highest plant stand was recorded with seed treatment of Penflufen 13.28% w/w + Trifloxystrobin 13.28% w/w (79.52%) compared with the untreated control (67.62%). Maximum grain yield was obtained in T8 (1310.53 kg ha⁻¹) followed by T7 (1278.87 kg ha⁻¹). Seed treatment combined with foliar application of fungicides also significantly improved plant growth parameters and seed weight over the untreated control. The study demonstrated that the integration of seed treatment with Penflufen + Trifloxystrobin and foliar application of Tebuconazole + Sulphur or Carbendazim + Mancozeb provides effective management of soybean anthracnose under field conditions.

References

[1]Agarwal DK, Billore SD, Sharma AN, Dupare BU and Srivastava SK. 2013. Soybean Introduction, Improvement, and utilization in India-problems and prospects. Agricultural Research 2(4): 293–300.

[2]Agam MN, Raut RA, Gb J and Sable SB. 2019. Evaluation of the fungicides, botanicals and bioagents against Colletotrichum truncatum causing anthracnose of soybean in pot culture. Journal of Pharmacognosy and Phytochemistry 8(2): 629–634.

[3]Amrate PK, Pancheshwar DK and Shrivastava MK. 2018. Application of fungicides and Trichoderma viride in management of soybean pod blight complex. International Journal of Chemical Studies 6(4): 3208–3210.

[4]Amrate PK, Shrivastava MK, Bhale MS, Agrawal N, Kumawat G, Shivkumar M and Nataraj V. 2023a. Identification and genetic diversity analysis of high yielding charcoal rot resistant soybean genotypes. Scientific Reports 13: 8905. https://doi.org/10.1038/s41598-023-35688-2

[5]Amrate PK, Shrivastava MK and Singh G. 2023b. Identification of Sources of Resistance and Yield Loss Assessment for Aerial Blight and Anthracnose/Pod Blight Diseases in Soybean. Legume Research 46(11): 1534–1540. doi:https://doi.org/10.18805/LR-4452

[6]Amrate PK. 2024. Survey and present status of soybean diseases in Central India. International Journal of Bio-resource and Stress Management 15(5): 01–10. https://doi.org/10.23910/1.2024.5299

[7]Banerjee J, Shrivastava MK, Singh Y and Amrate PK. 2023. Estimation of genetic divergence and proximate composition in advanced breeding lines of soybean (Glycine max (L.) Merrill). Environment and Ecology 41(3C): 1960–1968. https://doi.org/10.60151/envec/VYWE5744

[8]Bhatt P, Singh KP and Aravind T. 2022. Distribution and Identification of Colletotrichum species associated with soybean anthracnose/pod blight in different geographical locations of Uttarakhand. Legume Research 45(8): 1042–1047.

[9]FAOSTAT. 2023. Food and Agriculture Data. https://www.fao.org/

[10]Faske T, Kirkpatrick T, Zhou and Tzanetakis. 2014. Soybean diseases. Arkansas Soybean Production Handbook. pp. 2.

[11]Hosseini B, Voegele RT and Link TI. 2023. Diagnosis of Soybean Diseases caused by Fungal and Oomycete Pathogens: Existing Methods and New Developments. https://doi.org/10.3390/jof9050587

[12]Jeger MJ. 2004. Analysis of disease progress as a basis for evaluating disease management practices. Annual Review of Phytopathology 42: 61–82.

[13]Kale SL and Barhate BG. 2016. Management of anthracnose in soybean caused by Colletotrichum truncatum. International Journal of Plant Protection 9(2): 583–588.

[14]Kavanshree K, Jahagirdar S, Priyanka K, Uday G, Kambrekar DN, Krishnaraj PU, Basavaraja GT and Patil MS. 2023. Molecular variability of Colletotrichum spp. associated with anthracnose of soybean. Legume Research 45(8): 1048–1053.

[15]Khare MN and Chacko S. 1983. Factors affecting seed infection and transmission of Colletotrichum dematium f.sp. truncata in soybean. Seed Science and Technology 11: 853–858.

[16]Mandloi S, Jaiswal S, Rajput LS, Kumar S, Nataraj V, Shivkumar M, Maheswari HS, Sharma R, Pandey V and Bhatt J. 2023. In vitro evaluation of fungicides against Colletotrichum truncatum causing anthracnose of soybean. Soybean Research 21(1): 1–13.

[17]Mayee CD and Datar VV. 1986. Phytopathometry. Technical Bulletin No. 1 (Special Bulletin No. 3). Marathwada Agricultural University, Parbhani. pp. 34–37.

[18]Nataraj V, Shivakumar M, Kumawat G, Gupta S, Rajput LS, Kumar S, Sharma AN and Bhatia VS. 2020. Genetic inheritance and identification of germplasm sources for anthracnose resistance in soybean [Glycine max (L.) Merr.]. Genetic Resources and Crop Evolution 67: 1449–1456.

[19]Raghuwanshi et al. 2025. Understanding Colletotrichum truncatum, a pathogen of soybean anthracnose, and in vitro efficacy of modern fungicides for its management. International Journal of Bio-resource and Stress Management 16(3): 01–10. https://doi.org/10.23910/1.2025.5932

[20]Rajput L, Nataraj V, Kumar S, Amrate PK, Jahagirdar S, Huilgol SN, Chakruno P, Singh A, Maranna S, Ratnaparkhe MB, Borah M, Singh KP, Gupta S and Khandekar N. 2021. WAASB index revealed stable resistance sources for soybean anthracnose in India. Journal of Agricultural Science 159(9–10): 710–720. doi:https://doi.org/10.1017/S0021859622000016

[21]Rajput LS, Kumar S, Nataraj V, Shivakumar M and Maheshwari Ghodki BS. 2022. Evaluation of novel fungicides for management of soybean anthracnose disease and yield loss estimation. Legume Research. doi:https://doi.org/10.18805/LR-4783

[22]Rana M, Singh Y and Srivastava S. 2020. In vivo evaluation of fungicides and biocontrol agents against anthracnose of sorghum. Plant Cell Biotechnology and Molecular Biology 21(59–60): 814.

[23]Sagarika M, Amrate PK, Yadav VK and Shrivastava MK. 2023. Exploring potential of new generation fungicides as seed dresser in combating early infection of Macrophomina phaseolina in soybean. Indian Phytopathology 76: 1045–1053. https://doi.org/10.1007/s42360-023-00680-3

[24]Sharma SK, Gupta GK and Ramteke R. 2011. Colletotrichum truncatum [(Schw.) Andrus & W.D. Moore], the causal agent of anthracnose of soybean [Glycine max (L.) Merrill]. Soybean Research 9: 31–52.

[25]Singhmanini A, Kotasthane AS, Agrawal T and Mahilang A. 2022. Characterization of Colletotrichum species associated with soybean pod blight in soybean growing districts of Chhattisgarh. Indian Phytopathology. doi:https://doi.org/10.1007/s42360-023-00593-1

[26]Subedi S, Gharti DB, Neupane S and Ghimire T. 2015. Management of anthracnose in soybean using fungicides. Journal of Nepal Agricultural Research Council 1: 29–32.

[27]Uikey S, Sharma S, Amrate PK and Shrivastava MK. 2022. Identification of rich oil-protein and disease resistance genotypes in soybean [Glycine max (L.) Merrill]. International Journal of Bio-resource and Stress Management 13(5): 497–506. https://doi.org/10.23910/1.2022.2478

[28]Wasule D, Ingle Y, Shingote P and Parlawar N. 2022. Combinatorial application of fungicides for management of anthracnose of soybean caused by Colletotrichum truncatum. The Pharma Innovation Journal 11(9): 2542–2547.

Abstract

Keywords

References